Sequential Assembly of the Functional Material Micropatterns on the Hydrogel Sheet for Constructing Skeletal Muscle Cell-based Assay System

The functional material micropatterns-assembled hydrogel sheet has been prepared by means of sequential transfer technique to construct the skeletal muscle cell-based assay system. We demonstrated to construct micropatterned synaptic connection between motor neurons-skeletal muscle cells using this technique. Besides, assembly of the non-biological functional materials has also advantage to confer additional functions to a hydrogel assay system. The fluorescent microbeads were assembled onto a hydrogel as sensing elements of contractile force and metabolic activity of skeletal muscle cells. These flexible sensor sheets would be useful to study the relationship between exercise and metabolic activity of muscle in type 2 diabetes.